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Picture of an SDS-PAGE. The molecular markers (ladder) are in the left lane. Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.
SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa.
Proteins separated by SDS-PAGE, Coomassie brilliant blue staining. Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium, namely agarose or polyacrylamide.
A duplex ADF scanner with tray that holds up to 120 sheets at a time, the DS-30000 Large-Format Document Scanner can capture scans as small as 2 x 2 inches (5.08 x 5.08cm) and as large as 12 x 17 ...
Separate just by mass is commonly achieved using SDS-PAGE. SDS denatures the proteins, breaks apart most complexes, and approximately equalizes the mass-to-charge ratios. SDS must be done as the second, perpendicular dimension, as it breaks apart complexes (rendering native PAGE impossible) and equalizes mass-to-charge ratios (rendering IEF ...
Duplex scanning document scanner (Fujitsu fi-5110C) Duplex scanning is usually implemented on multifunction printers using a Reversing Automatic Document Feeder (RADF), which removes, reverses, and re-feeds the document after scanning one side. Duplex scanning is achieved on scanners by either RADF or by single pass duplex scanning using two ...
Document imaging is an information technology category for systems capable of replicating documents commonly used in business. Document imaging systems can take many forms including microfilm, on demand printers, facsimile machines, copiers, multifunction printers, document scanners, computer output microfilm (COM) and archive writers.
The three samples are mixed and loaded onto IEF (isoelectric focusing chromatography) for first dimension and the strip is transferred to a SDS PAGE.After the gel electrophoresis, the gel is scanned with the excitation wavelength of each dye one after the other, so each sample can be seen separately (if we scan the gel at the excitation wavelength of the Cy3 dye, we will see in the gel only ...