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Optical units are dimensionless units of length used in optical microscopy. They are used to express distances in terms of the numerical aperture of the system and the wavelength of the light used for observation. Using these units allows comparison of the properties of different microscopes. [1]
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
The single lens with its attachments, or the system of lenses and imaging equipment, along with the appropriate lighting equipment, sample stage, and support, makes up the basic light microscope. The most recent development is the digital microscope, which uses a CCD camera to focus on the exhibit of interest. The image is shown on a computer ...
Modern microscopes, known as compound microscopes have many lenses in them (typically four) to optimize the functionality and enhance image stability. [111] A slightly different variety of microscope, the comparison microscope, looks at side-by-side images to produce a stereoscopic binocular view that appears three dimensional when used by ...
Daily experiences of matter and the Universe are characterized by very low energy. For example, the photon energy of visible light is about 1.8 to 3.2 eV. Similarly, the bond-dissociation energy of a carbon-carbon bond is about 3.6 eV. This is the energy scale manifesting at the macroscopic level, such as in chemical reactions
The sub-stage condenser focuses light through the specimen to match the aperture of the objective lens system. The Abbe condenser is named for its inventor Ernst Abbe , who developed it in 1870. The Abbe condenser, which was originally designed for Zeiss, is mounted below the stage of the microscope.
The shape and texture in each individual grain is made visible through the microscope. [7] As the microscopic scale covers any object that cannot be seen by the naked eye, yet is visible under a microscope, the range of objects that fall under this scale can be as small as an atom, visible underneath a transmission electron microscope. [8]
The wave nature of light limits the size of the spot to which light can be focused due to the diffraction limit. This limitation was described in the 19th century by Ernst Abbe and "limits an optical microscope's resolution to approximately half of the wavelength of the light used." Fluorescence microscopy is central to many techniques which ...