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This method is a faster process than dry heat sterilization. Steam sterilization is performed using an autoclave, sometimes called a converter or steam sterilizer. The object or liquid is placed in the autoclave chamber, which is then sealed and heated using pressurized steam to a temperature set point for a defined period of time.
Sterilization can also be achieved using ethylene oxide (ETO) gas. This process was created in the 1950s by the US military [4] and is used on items that cannot withstand the high temperatures of steam sterilization. ETO sterilization takes far longer than steam sterilization and is hazardous to workers, so alternative methods were created in ...
An autoclave, which uses steam under pressure, is used in microbiology laboratories and similar environments for sterilization. Steam, especially dry (highly superheated) steam, may be used for antimicrobial cleaning even to the levels of sterilization. Steam is a non-toxic antimicrobial agent. [11] [12]
A vacuum pump sucks air or air/steam mixtures from the chamber. Superatmospheric cycles: Achieved with a vacuum pump. It starts with a vacuum followed by a steam pulse followed by a vacuum followed by a steam pulse. The number of pulses depends on the particular autoclave and cycle chosen. Subatmospheric cycles:
The time is counted once the temperature that is needed has been reached. Steam sterilization requires four conditions in order to be efficient: adequate contact, sufficiently high temperature, correct time and sufficient moisture. [16] Sterilization using steam can also be done at a temperature of 132 C (270 F), at a double pressure. [citation ...
In 1953, APV pioneered a steam injection technology, involving direct injection of steam through a specially designed nozzle which raises the product temperature instantly, under brand name Uperiser; milk was packaged in sterile cans. In the 1960s, APV launched the first commercial steam infusion system under the Palarisator brand name. [7] [8]
Sterilization may refer to: Sterilization (microbiology), killing or inactivation of micro-organisms; Soil steam sterilization, a farming technique that sterilizes soil with steam in open fields or greenhouses; Sterilization (medicine) renders a human unable to reproduce; Neutering is the surgical sterilization of animals
Tyndallization is a process from the nineteenth century for sterilizing substances, usually food, named after its inventor John Tyndall, that can be used to kill heat-resistant endospores.