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The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
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Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]
Many dogs with house dust mite allergy have perennial disease. [4] Some of the allergens associated with atopy in dogs include pollens of trees, grasses and weeds, as well as molds and house dust mites. Ear and skin infections by the bacteria Staphylococcus pseudintermedius and the yeast Malassezia pachydermatis are commonly secondary to atopic ...
Saccharomyces cerevisiae (/ ˌ s ɛr ə ˈ v ɪ s i. iː /) (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungal microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes.
In yeast, deletion strains are frequently used to assess protein stability over time with cycloheximide chases. For example, yeast strains lacking critical degradation machinery such as chaperones, E3 ligases, and vacuolar proteins are often used to determine the mechanism of degradation for a protein substrate of interest.
Malassezia pachydermatis is a zoophilic yeast in the division Basidiomycota.It was first isolated in 1925 by Fred Weidman, and it was named pachydermatis (Greek for 'thick-skin') after the original sample taken from an Indian rhinoceros (Rhinocerosus unicornis) with severe exfoliative dermatitis. [2]
This system has allowed researchers to manipulate a variety of genetically modified organisms to control gene expression, delete undesired DNA sequences and modify chromosome architecture. The Cre protein is a site-specific DNA recombinase that can catalyse the recombination of DNA between specific sites in a DNA molecule.
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