Search results
Results from the WOW.Com Content Network
The Isolation chip (or ichip) is a method of culturing bacteria. Using regular methods, 99% of bacterial species are not able to be cultured as they do not grow in conditions made in a laboratory, a problem called the "Great Plate Count Anomaly". [1] The ichip instead cultures bacterial species within its soil environment.
Gram-negative bacteria will stain a pink color due to the thin layer of peptidoglycan. If a bacteria stains purple, due to the thick layer of peptidoglycan, the bacteria is a gram-positive bacteria. [4] In clinical microbiology numerous other staining techniques for particular organisms are used (acid fast bacterial stain for mycobacteria).
Fast ChIP (qChIP): The fast ChIP assay reduced the time by shortening two steps in a typical ChIP assay: (i) an ultrasonic bath accelerates the rate of antibody binding to target proteins—and thereby reduces immunoprecipitation time (ii) a resin-based (Chelex-100) DNA isolation procedure reduces the time of cross-link reversal and DNA isolation.
Bacteria, which can thrive in places where humans wouldn't dare linger, can be friends or foes. Take E. coli. Some strains are harmless and settle comfortably in animals' lower intestines. Others ...
TSA plates support growth of many semifastidious bacteria, including some species of Brucella, Corynebacterium, Listeria, Neisseria, and Vibrio. Xylose-lysine-deoxycholate agar is used for the culture of stool samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative bacilli is ...
Negative selection through replica plating to screen for ampicillin sensitive colonies. Replica plating is a microbiological technique in which one or more secondary Petri plates containing different solid (agar-based) selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) are inoculated with the same colonies of microorganisms from a primary ...
Off-chip HPLC columns are used to separate analytes before feeding them into a microfluidic device for fractionation and analysis. [214] HPLC columns can also be built directly into microfluidic lab-chips creating monolithic hybrid devices capable of chemical separation as well as droplet formation and manipulation.
Swarming Serratia marcescens cells were transferred to PDMS-coated coverslips, resulting in a structure referred to as a "bacterial carpet" by the authors. Differently shaped flat fragments of this bacterial carpets, termed "auto-mobile chips", moved above the surface of the microscope slide in two dimensions. [42]