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The plaque reduction neutralization test is used to quantify the titer of neutralizing antibody for a virus. [1] [2] The serum sample or solution of antibody to be tested is diluted and mixed with a viral suspension. This is incubated to allow the antibody to react with the virus. This is poured over a confluent monolayer of host cells.
An MSA plate with Micrococcus sp. (1), Staphylococcus epidermidis (2) and S. aureus colonies (3). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [1]
The plates are incubated for 12 hours up to several days, depending on the test that is performed. Commonly used types of agar plates include: Red blood cells on an agar plate are used to diagnose infection. On the left is a positive Staphylococcus infection, on the right a positive Streptococcus culture.
Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery , [ 1 ] bioassay validation, quality control and manufacturing processes in the pharmaceutical and ...
As an analytical biochemistry assay and a "wet lab" technique, ELISA involves detection of an analyte (i.e., the specific substance whose presence is being quantitatively or qualitatively analyzed) in a liquid sample by a method that continues to use liquid reagents during the analysis (i.e., controlled sequence of biochemical reactions that will generate a signal which can be easily ...
In the biological sciences, a multiplex assay is a type of immunoassay that uses magnetic beads to simultaneously measure multiple analytes in a single experiment. [1] A multiplex assay is a derivative of an ELISA using beads for binding the capture antibody.
Microtiter plates with 96, 384 and 1536 wells. A microplate, also known as a microtiter plate, microwell plate or multiwell, [1] is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories.
Petri dish/agar plate: to act as a supporting container to hold the culture medium in Platinum wire loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; sterilized by flaming to red hot before use Pre-sterilized disposable container: specimen collection