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The swarming capability of Proteus mirabilis is important because it is implicated in the pathogenesis of the bacteria and the swarming capability is associated with the bacteria's ability to express virulence factors [9] Proteus mirabilis has a very characteristic bulls-eye appearance on an agar plate due to the regular periodic cycling ...
Nutrient gelatin tests to detect activity of the enzyme gelatinase. In a clinical setting, three species make up 80 to 95% of all isolates identified. These are Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. However, Proteus mirabilis is now considered a part of the Morganellaceae, a sister clade within the Enterobacterales.
Among these are Pseudomonas aeruginosa, which has a grape-like scent; Staphylococcus aureus, which is said to smell like old socks; and Proteus mirabilis, whose scent is alternately described as putrid [1]: 168 or like chocolate cake. [7]: 124 Other distinctive features of colonial morphology include motility and the production of pigments.
Proteus species do not usually ferment lactose. Similar to other members of the Enterobacterales order, bacteria from the Proteus genus are glucose fermenting, oxidase-negative, catalase-positive, and nitrate-positive. Glucose fermentation in this species can be demonstrated through the triple sugar iron test.
P. rettgeri can be incubated at 37 °C in nutrient agar or nutrient broth. It was first discovered in 1904 after a waterfowl epidemic. [1] Strains of the species have also been isolated from nematodes of the genus Heterorhabditis. [2] Providencia rettgeri also found in marine environment. [3]
One particular feature of this type of motility is the formation of dendritic fractal-like patterns formed by migrating swarms moving away from an initial location. Although the majority of species can produce tendrils when swarming, some species like Proteus mirabilis do form concentric circles motif instead of dendritic patterns. [19]
Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): [1] 0.5% peptone – this provides organic nitrogen; 0.3% beef extract/yeast extract – the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts; 1.5% agar ...
Although it could be used in other formats (e.g., Petri plates), Simmons' citrate agar is often used in slants or slopes in test tubes. One advantage of using slants over Petri dishes is access to oxygen, which is required by citrate metabolism. Simmons’ agar can be bought from suppliers as ready-made powders or slants.