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A cell line can be defined as a permanently established cell culture which will propagate forever. Investigators mostly get cell lines from other investigators or from cell banks (such as the American Type Culture Collection) , because its much easier than creating new one. In special cases, investigators are obligated to establish a cell line.
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and/or increase the number of cells or microorganisms in the culture. [1]
For example, microorganisms or cells can be studied in artificial culture media, and proteins can be examined in solutions. Colloquially called "test-tube experiments", these studies in biology, medicine, and their subdisciplines are traditionally done in test tubes, flasks, Petri dishes, etc. [ 6 ] [ 7 ] They now involve the full range of ...
Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture compartment at the desired cell ratio. [47] Cells can communicate by paracrine and juxtacrine signaling, but separated treatments and downstream analysis of a single cell type are not readily feasible due to the completely ...
Light micrograph of a moss's leaf cells at 400X magnification. The following outline is provided as an overview of and topical guide to cell biology: . Cell biology – A branch of biology that includes study of cells regarding their physiological properties, structure, and function; the organelles they contain; interactions with their environment; and their life cycle, division, and death.
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Obtaining a culture with a unified cell-cycle stage is useful for biological research where a particular stage in the cell cycle is desired (such as the culturing of parasitized cells [3]). Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be ...