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In optics and lens design, the Abbe number, also known as the Vd-number or constringence of a transparent material, is an approximate measure of the material's dispersion (change of refractive index versus wavelength), with high values of Vd indicating low dispersion. It is named after Ernst Abbe (1840–1905), the German physicist who defined it.
Memorial in Jena, Germany to Ernst Karl Abbe, who approximated the diffraction limit of a microscope as = , where d is the resolvable feature size, λ is the wavelength of light, n is the index of refraction of the medium being imaged in, and θ (depicted as α in the inscription) is the half-angle subtended by the optical objective lens (representing the numerical aperture).
The focus of the first lens is traditionally about 2mm away from the plane face coinciding with the sample plane. A pinhole cap can be used to align the optical axis of the condenser with that of the microscope. The Abbe condenser is still the basis for most modern light microscope condenser designs, even though its optical performance is poor.
In optics, the Abbe sine condition is a condition that must be fulfilled by a lens or other optical system in order for it to produce sharp images of off-axis as well as on-axis objects. It was formulated by Ernst Abbe in the context of microscopes. [1] The Abbe sine condition says that. the sine of the object-space angle should be proportional ...
In optics, chromatic aberration (CA), also called chromatic distortion, color aberration, color fringing, or purple fringing, is a failure of a lens to focus all colors to the same point. [1][2] It is caused by dispersion: the refractive index of the lens elements varies with the wavelength of light. The refractive index of most transparent ...
Near-field scanning optical microscopy (NSOM) or scanning near-field optical microscopy (SNOM) is a microscopy technique for nanostructure investigation that breaks the far field resolution limit by exploiting the properties of evanescent waves. In SNOM, the excitation laser light is focused through an aperture with a diameter smaller than the ...
In Abbe's 1874 paper, titled "A Contribution to the Theory of the Microscope and the nature of Microscopic Vision", [12] Abbe states that the resolution of a microscope is inversely dependent on its aperture, but without proposing a formula for the resolution limit of a microscope. In 1876, Abbe was offered a partnership by Zeiss and began to ...
STED microscopy is one of several types of super resolution microscopy techniques that have recently been developed to bypass the diffraction limit of light microscopy to increase resolution. STED is a deterministic functional technique that exploits the non-linear response of fluorophores commonly used to label biological samples in order to ...