Search results
Results from the WOW.Com Content Network
Ames test procedure. The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. More formally, it is a biological assay to assess the mutagenic potential of chemical compounds. [1]
Ames made contributions to understanding the mechanisms of mutagenesis and DNA repair. He invented the Ames test, a widely used assay for easily and cheaply evaluating the mutagenicity of compounds. [3] The test revolutionized the field of toxicology and has played a crucial role in identifying numerous environmental and industrial carcinogens.
A young Ames in the 1958 McLean High School yearbook. Ames was born in River Falls, Wisconsin, on May 26, 1941, to Carleton Cecil Ames and Rachel Ames (née Aldrich).His father was a college lecturer at the Wisconsin State College-River Falls, and his mother a high school English teacher.
Ames test procedure. One classical bioassay is the Ames test. A strain of Salmonella that requires histidine to grow is put on two plates with growth medium containing minimal amounts of histidine and some rat liver extract (to mimick liver metabolism). A suspected mutagen is added to one plate. If the plate with the suspected mutagen grows ...
The Ames Research Center (ARC), also known as NASA Ames, is a major NASA research center at Moffett Federal Airfield in California's Silicon Valley. It was founded in 1939 [ 1 ] as the second National Advisory Committee for Aeronautics (NACA) laboratory.
The S9 fraction has been used in conjunction with the Ames test [4] to assess the mutagenic potential of chemical compounds. [5] Chemical substances sometimes require metabolic activation in order to become mutagenic. Furthermore, the metabolic enzymes of bacteria used in the Ames test differ substantially from those in mammals.
A chemical is considered positive for Ames test if it causes mutations increasing the observed reversion rate and negative if presents similar to the control group. There is a normal, but small, number of revertant colonies expected when an auxotrophic bacteria is plated on a media without the metabolite it needs because it could mutate back to ...
The high correlation between the Umu Chromotest and traditional Ames test for mutagenicity supports it as a reasonable alternative for early-stage testing of the thousands of new pharmaceutical, agricultural and industrial chemicals synthesized every year. Most large chemical manufacturers have the ability to screen 100 or more synthetic ...