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Primer extension offers an alternative to a nuclease protection assay (S1 nuclease mapping) for quantifying and mapping RNA transcripts. The hybridization probe for primer extension is a synthesized oligonucleotide, whereas S1 mapping requires isolation of a DNA fragment. Both methods provide information where a mRNA starts and provide an ...
Detonating cord (also called detonation cord, detacord, detcord, blasting rope, or primer cord) is a thin, flexible plastic tube usually filled with pentaerythritol tetranitrate (PETN, pentrite). With the PETN exploding at a rate of approximately 6,400 m/s (21,000 ft/s), any common length of detonation cord appears to explode instantaneously.
The toeprinting assay, also known as the primer extension inhibition assay, [1] is a method used in molecular biology that allows one to examine the interactions between messenger RNA and ribosomes or RNA-binding proteins. [2] It is different from the more commonly used DNA footprinting assay. The toeprinting assay has been utilized to examine ...
Yellow NEMA 5-15 extension cord NEMA-1 extension cord, common in the United States Extension cord reel (Germany). An extension cord (US), extension cable, power extender, drop cord, or extension lead (UK) is a length of flexible electrical power cable (flex) with a plug on one end and one or more sockets on the other end (usually of the same type as the plug).
Primer extension is a two step process that first involves the hybridization of a probe to the bases immediately upstream of the SNP nucleotide followed by a ‘mini-sequencing’ reaction, in which DNA polymerase extends the hybridized primer by adding a base that is complementary to the SNP nucleotide.
In the method, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid to form a duplex, with the primer’s terminal 3’-end directly adjacent to the nucleotide base to be identified. Using a DNA polymerase, the oligonucleotide primer is enzymatically extended by a single base in the presence of all four ...
Solid core cable is intended for permanently installed runs (permanent link). It is less flexible than stranded cable and is more prone to failure if repeatedly flexed due to work hardening. Stranded cable is used at patch panels and for connections from wall ports to end devices (patch cord or drop cable), as it resists cracking of the conductors.
The primer is allowed to extend one base pair into the C (or T) using DNA polymerase terminating dideoxynucleotides, and the ratio of C to T is determined quantitatively. A number of methods can be used to determine this C:T ratio. At the beginning, MS-SnuPE relied on radioactive ddNTPs as the reporter of the