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Micrograph of paper autofluorescing under ultraviolet illumination. The individual fibres in this sample are around 10 μm in diameter.. Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores).
These fluorescence can result in artifacts or background noise in the image. Noise from other fluorescence compounds can be effectively eliminated by using filters to capture the desired fluorescence wavelength, or by making sure the autofluorescence compounds are not present in the sample.
Other problems that may arise when using immunofluorescence techniques include autofluorescence, spectral overlap and non-specific staining. [1] [2] Autofluorescence includes the natural fluorescence emitted from the sample tissue or cell itself. Spectral overlap happens when a fluorophore has a broad emission specter, that overlaps with the ...
Extraction Profile for Different Types of Extraction. The extraction curve of % recovery against time can be used to elucidate the type of extraction occurring. Figure 4(a) shows a typical diffusion controlled curve. The extraction is initially rapid, until the concentration at the surface drops to zero, and the rate then becomes much slower.
In autofluorescence, fluorescence from the eye occurs without injection of the dye. This may be seen with optic nerve head drusen , astrocytic hamartoma , or calcific scarring. Black-and-white photos give better contrast than color photos, which aren't necessary because the filter transmits only one color of light.
Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix. The distribution of a solute between two phases is an equilibrium condition described by partition theory.
WASHINGTON (Reuters) -The Federal Aviation Administration and National Transportation Safety Board plan to brief senators Thursday on last week's midair collision between an American Airlines ...
Solid-phase extraction (SPE) [1] is a solid-liquid extractive technique, by which compounds that are dissolved or suspended in a liquid mixture are separated, isolated or purified, from other compounds in this mixture, according to their physical and chemical properties. Analytical laboratories use solid phase extraction to concentrate and ...