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Microtechnique is an aggregate of methods used to prepare micro-objects for studying. [1] It is currently being employed in many fields in life science. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique.
Current Protocols is a series of laboratory manuals for life scientists. The first title, Current Protocols in Molecular Biology, was established in 1987 by the founding editors Frederick M. Ausubel, Roger Brent, Robert Kingston, David Moore, Jon Seidman, Kevin Struhl, and John A. Smith of the Massachusetts General Hospital Department of Molecular Biology and the Harvard Medical School ...
With most types of plant stress, this variable fluorescence can be used to measure the level of plant stress. The most commonly used protocols include: Fv/Fm, a dark adapted protocol, Y(II) or ΔF/Fm’ a light adapted test that is used during steady state photosynthesis, and various OJIP, dark adapted protocols that follow different schools of ...
Y(II) is a measuring protocol that was developed by Bernard Genty with the first publications in 1989 and 1990. [12] [13] It is a light adapted test that allows one to measure plant stress while the plant is undergoing the photosynthetic process at steady-state photosynthesis lighting conditions. Like FvFm, Y(II) represents a measurement ratio ...
In fact, such predefined protocols are an essential component of Good Laboratory Practice (GLP) [11] and Good Clinical Practice (GCP) [12] [13] regulations. Protocols written for use by a specific laboratory may incorporate or reference standard operating procedures (SOP) governing general practices required by the laboratory. A protocol may ...
For instance, in a study on Arabidopsis thaliana, biologically important regions of the plant's genome were found to be protected from mutations, and beneficial mutations were found to be more likely, i.e. mutation was "non-random in a way that benefits the plant". [146] [147] [148]
The EPA's Good Laboratory Practice Standards (GLPS) compliance monitoring program guarantees the accuracy and reliability of test data submitted to the Agency to support pesticide product registration under the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA), section 5 of the Toxic Substances Control Act (TSCA), and in accordance ...
Genetically modified crops (GM crops) are plants used in agriculture, the DNA of which has been modified using genetic engineering methods. Plant genomes can be engineered by physical methods or by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors.