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Only the duplex without overlap at the 5' end will allow extension by DNA polymerase in 3' to 5' direction. Following the extension of the OE-PCR reaction, the PCR mix or the eluted fragments of appropriate size are subject to normal PCR, using the outermost primers used in the initial, mutagenic PCR reactions.
Each packing problem has a dual covering problem, which asks how many of the same objects are required to completely cover every region of the container, where objects are allowed to overlap. In a bin packing problem, people are given: A container, usually a two- or three-dimensional convex region, possibly of infinite size. Multiple containers ...
Conversion and its related terms yield and selectivity are important terms in chemical reaction engineering.They are described as ratios of how much of a reactant has reacted (X — conversion, normally between zero and one), how much of a desired product was formed (Y — yield, normally also between zero and one) and how much desired product was formed in ratio to the undesired product(s) (S ...
The overlap coefficient, [note 1] or Szymkiewicz–Simpson coefficient, [citation needed] [3] [4] [5] is a similarity measure that measures the overlap between two finite sets.It is related to the Jaccard index and is defined as the size of the intersection divided by the size of the smaller of two sets:
The most widely used gap penalty function is the affine gap penalty. The affine gap penalty combines the components in both the constant and linear gap penalty, taking the form + (). This introduces new terms, A is known as the gap opening penalty, B the gap extension penalty and L the length of the gap.
The bird flu outbreak continues to grow in the U.S., with state officials confirming infected flocks in two additional Michigan counties on Monday. Following an investigation by the Michigan ...
Unidirectional or tandem overlap: the 3' end of one gene overlaps with the 5' end of another gene on the same strand. This arrangement can be symbolized with the notation → → where arrows indicate the reading frame from start to end. Convergent or end-on overlap: the 3' ends of the two genes overlap on opposite strands. This can be written ...
The test also measured incidents of hypopneas, ... Upon analysis, Ramos and his team found that participants with the most sleeping issues had 0.24 cubic centimeters (cm 3) ...