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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
PowerBooter is an automated power model that uses built-in battery voltage sensors and behavior of battery during discharge to monitor power consumption of total system. This method does not require any especial external measurement equipment. PowerTutor is also a power measurement tool that uses PowerBooter generated data for online power ...
Its production may be induced by a non-hydrolyzable analog of allolactose, IPTG, which binds and releases the lac repressor from the lac operator, thereby allowing the initiation of transcription to proceed. It is commonly used in molecular biology as a reporter marker to monitor gene expression.
The video’s popularity sparked a conversation about valuing postal workers and other service industry professionals. Vaughan, who worked in the service industry, says: “I think everyone should ...
A CPU cache is a piece of hardware that reduces access time to data in memory by keeping some part of the frequently used data of the main memory in a 'cache' of smaller and faster memory. The performance of a computer system depends on the performance of all individual units—which include execution units like integer, branch and floating ...
The NFL said in a statement that it "will continue to closely monitor developments" and will "remain in contact with public officials, both clubs and the NFLPA." On Thursday, ...
4 top factors that affect your mortgage rate. The difference of even half a percentage point on your interest rate can save you hundreds of dollars a month and thousands of dollars over the life ...