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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
Charge trap flash (CTF) is a semiconductor memory technology used in creating non-volatile NOR and NAND flash memory. It is a type of floating-gate MOSFET memory technology , but differs from the conventional floating-gate technology in that it uses a silicon nitride film to store electrons rather than the doped polycrystalline silicon typical ...
IPTG, a molecule similar to lactose, but with a sulfur bond that is not hydrolyzable so that E. coli does not digest it, is used to activate or "induce" the production of the new protein. Once the cells are induced, it is difficult to remove IPTG from the cells and therefore it is difficult to stop expression.
The phase of the Moon does not influence fertility, cause a fluctuation in crime, or affect the stock market. There is no correlation between the lunar cycle and human biology or behavior. However, the increased amount of illumination during the full moon may account for increased epileptic episodes, motorcycle accidents, or sleep disorders.
The video’s popularity sparked a conversation about valuing postal workers and other service industry professionals. Vaughan, who worked in the service industry, says: “I think everyone should ...
Certain developments in computer technology and business needs have tended to increase the relative advantages of in-memory technology. [11] Following Moore's law, the number of transistors per square unit doubles every two or so years. This is reflected in changes to price, performance, packaging and capabilities of the components.
Steve Guttenberg hit the ground running to help people impacted by the fires in Pacific Palisades — and he was almost unrecognizable. The flames first began around 10:30 a.m. local time on ...