Search results
Results from the WOW.Com Content Network
The quinone is red-violet in colour, [3] [5] with the intensity of the colour being in proportion to the glucose concentration. [3] The colour is measured at 505 nm, [2] 510 nm, [4] or 540 nm. [3] Diagnostic kits containing the Trinder reagent are available, including one from Sigma-Aldrich. [2]
The glucose oxidase enzyme (GOx or GOD) also known as notatin (EC number 1.1.3.4) is an oxidoreductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present.
3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (). [1]
Sigma Advanced Genetic Engineering (SAGE) Labs is a division within Sigma-Aldrich that specializes in genetic manipulation of in vivo systems for special research and development applications. It was formed in 2008 to investigate zinc finger nuclease technology and its application for disease research models.
If the medium in the anaerobic tube turns yellow, then the bacteria are fermenting glucose. If the tube with oil doesn't turn yellow, but the open tube does turn yellow, then the bacterium is oxidizing glucose. If the tube with mineral oil doesn't change, and the open tube turns blue, then the organism neither ferments, nor oxidizes glucose.
The oxidase test is used to determine whether an organism possesses the cytochrome c oxidase enzyme. The test is used as an aid for the differentiation of Neisseria , Moraxella , Campylobacter and Pasteurella species (oxidase positive).
In biochemistry, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) is a chemical compound used to observe the reaction kinetics of specific enzymes.A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect the binding of molecules to each other.
Cellobiose is a disaccharide with the formula (C 6 H 7 (OH) 4 O) 2 O. It is classified as a reducing sugar - any sugar that possesses the ability or function of a reducing agent. The chemical structure of cellobiose is derived from the condensation of a pair of β-glucose molecules forming a β(1→4) bond.