Search results
Results from the WOW.Com Content Network
Atomic Fluorescence Spectroscopy (AFS) techniques are useful in other kinds of analysis/measurement of a compound present in air or water, or other media, such as CVAFS which is used for heavy metals detection, such as mercury. Fluorescence can also be used to redirect photons, see fluorescent solar collector.
Chlorophyll fluorescence is a widely-used proxy for the quantity (biomass) of microscopic algae in the water. In the lab after water sampling, researchers extract the pigments out of a filter that has phytoplankton cells on it, then measure the fluorescence of the extract in a benchtop fluorometer in a dark room. [7]
Fluorescence in the life sciences is used generally as a non-destructive way of tracking or analysis of biological molecules by means of the fluorescent emission at a specific frequency where there is no background from the excitation light, as relatively few cellular components are naturally fluorescent (called intrinsic or autofluorescence).
Chlorophyll fluorometers are designed to measure variable fluorescence of photosystem II. This variable fluorescence can be used to measure the level of plant stress. The most commonly used protocols include those aimed at measuring the photosynthetic efficiency of photosystem II, both in the light (ΔF/Fm') and in a dark-adapted state (Fv/Fm ...
The chloride ion is a well known quencher for quinine fluorescence. [2] [3] [4] Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence. Quenching is made use of in optode sensors; for instance the quenching effect of oxygen on certain ruthenium complexes allows the measurement of oxygen saturation in
Schematic diagram of the arrangement of optical components in a typical Spectrofluorometer. A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample.
Surface water loss; Limitations of use in acidic waters; Depending on the environment, water flows possess certain factors that can affect how a dye performs. Natural fluorescence in a water flow can interfere with certain dyes. The presence of organic material, other chemicals, and sunlight can affect the intensity of dyes.
In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed. The fluorescence intensity is fluctuating due to Brownian motion of the particles. In other words, the number of the particles in the sub-space defined by the optical system is randomly ...