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TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.. Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not ...
Dithiobutylamine (DTBA) is a reducing agent intended as an alternative for DTT in biochemical uses. It was designed to be easily synthesized in non-racemic form, to have a lower pKa (allowing more effective reduction at neutral pH), and to have a low disulfide E°′ reduction potential. [1]
Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol . DTT is redox reagent also known as Cleland's reagent , after W. Wallace Cleland . [ 2 ]
2-Mercaptoethanol is often used interchangeably with dithiothreitol (DTT) or the odorless tris(2-carboxyethyl)phosphine (TCEP) in biological applications. Although 2-mercaptoethanol has a higher volatility than DTT, it is more stable: 2-mercaptoethanol's half-life is more than 100 hours at pH 6.5 and 4 hours at pH 8.5; DTT's half-life is 40 ...
Piping and instrumentation diagram of pump with storage tank. Symbols according to EN ISO 10628 and EN 62424. A more complex example of a P&ID. A piping and instrumentation diagram (P&ID) is defined as follows: A diagram which shows the interconnection of process equipment and the instrumentation used to control the process.
A control valve is a valve used to control fluid flow by varying the size of the flow passage as directed by a signal from a controller. [1] This enables the direct control of flow rate and the consequential control of process quantities such as pressure, temperature, and liquid level.
An isolation valve is a valve in a fluid handling system that stops the flow of process media to a given location, usually for maintenance or safety purposes. [1] They can also be used to provide flow logic (selecting one flow path versus another), and to connect external equipment to a system. [ 2 ]
Choke valve, Butterfly valve used to limit air intake in internal combustion engine. (Not to be confused with choke valves used in industrial flow control, above.) Clapper valve: a type of check valve used in the Siamese fire appliance to allow only one hose to be connected instead of two (the clapper valve blocks the other side from leaking out)