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Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The lifespan of most cells is genetically determined, but some cell-culturing cells have been 'transformed' into immortal cells which will reproduce indefinitely if the optimal conditions are provided.
The third method is cell culture, of which there are three types: (1) precursor cell culture, i.e. undifferentiated cells that are to be differentiate, (2) differentiated cell culture, i.e. completely differentiated cells that have lost the capacity to further differentiate, and (3) stem cell culture, i.e. undifferentiated cells that can ...
A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. [1]
Line of T175 cell culture flasks containing red cell culture medium. In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and ...
Cell culture in a special tissue culture dish. Cell culture can be an alternative to animal use in some cases. For example, cultured cells have been developed to create monoclonal antibodies; prior to this, production required animals to undergo a procedure likely to cause pain and distress. [7]
To get a detailed description of the various methods see protein methods and nucleic acid methods. Besides the aforementioned groups of techniques, biotechnology also includes plant tissue culture methods, animal cell culture methods, and microbial fermentation methods.
Dilution cloning or cloning by limiting dilution [1] [2] describes a procedure to obtain a monoclonal cell population starting from a polyclonal mass of cells. This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. A suspension of the parent cells is made. Appropriate dilutions are then made ...
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. [1]