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  2. Transparency and translucency - Wikipedia

    en.wikipedia.org/wiki/Transparency_and_translucency

    An object may be not transparent either because it reflects the incoming light or because it absorbs the incoming light. Almost all solids reflect a part and absorb a part of the incoming light. When light falls onto a block of metal , it encounters atoms that are tightly packed in a regular lattice and a " sea of electrons " moving randomly ...

  3. Opacity - Wikipedia

    en.wikipedia.org/wiki/Opacity

    An opaque object is neither transparent (allowing all light to pass through) nor translucent (allowing some light to pass through). When light strikes an interface between two substances, in general, some may be reflected, some absorbed, some scattered, and the rest transmitted (also see refraction).

  4. Phase-contrast microscopy - Wikipedia

    en.wikipedia.org/wiki/Phase-contrast_microscopy

    Phase-contrast microscopy is particularly important in biology. It reveals many cellular structures that are invisible with a bright-field microscope , as exemplified in the figure. These structures were made visible to earlier microscopists by staining , but this required additional preparation and death of the cells.

  5. Light - Wikipedia

    en.wikipedia.org/wiki/Light

    A transparent object allows light to transmit or pass through. Conversely, an opaque object does not allow light to transmit through and instead reflecting or absorbing the light it receives. Most objects do not reflect or transmit light specularly and to some degree scatters the incoming light, which is called glossiness .

  6. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Rheinberg illumination is a variant of dark field illumination in which transparent, colored filters are inserted just before the condenser so that light rays at high aperture are differently colored than those at low aperture (i.e., the background to the specimen may be blue while the object appears self-luminous red). Other color combinations ...

  7. Invisibility - Wikipedia

    en.wikipedia.org/wiki/Invisibility

    By using two parabolic cylindric mirrors and one plane mirror, the image of the background is directed around an object, making the object itself invisible - at least from two sides. Invisibility is the state of an object that cannot be seen. An object in this state is said to be invisible (literally, "not visible").

  8. Tissue clearing - Wikipedia

    en.wikipedia.org/wiki/Tissue_clearing

    [1] [10] In the 1980s, Andrew Murray & Marc Kirschner developed a two-step process, wherein tissues were first dehydrated with alcohol and subsequently made transparent by immersion in a mixture of benzyl alcohol and benzyl benzoate (BABB), a technique they coupled with light sheet fluorescence microscopy, [11] [2] [3] which remains the method ...

  9. Live-cell imaging - Wikipedia

    en.wikipedia.org/wiki/Live-cell_imaging

    To keep cells alive during observation, the microscopes are commonly enclosed in a micro cell incubator (the transparent box). Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1]