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  2. Phase-contrast microscopy - Wikipedia

    en.wikipedia.org/wiki/Phase-contrast_microscopy

    Phase-contrast microscopy is particularly important in biology. It reveals many cellular structures that are invisible with a bright-field microscope, as exemplified in the figure. These structures were made visible to earlier microscopists by staining, but this required additional preparation and death of the cells. The phase-contrast ...

  3. Live-cell imaging - Wikipedia

    en.wikipedia.org/wiki/Live-cell_imaging

    [20] [21] Quantitative phase-contrast microscopy has an advantage over fluorescent and phase-contrast microscopy in that it is both non-invasive and quantitative in its nature. Due to the narrow focal depth of conventional microscopy, live-cell imaging is to a large extent currently limited to observing cells on a single plane.

  4. Watershed (image processing) - Wikipedia

    en.wikipedia.org/wiki/Watershed_(image_processing)

    A function W is a watershed of a function F if and only if W ≤ F and W preserves the contrast between the regional minima of F; where the contrast between two regional minima M 1 and M 2 is defined as the minimal altitude to which one must climb in order to go from M 1 to M 2. [7] An efficient algorithm is detailed in the paper. [8] Watershed ...

  5. Phase-contrast imaging - Wikipedia

    en.wikipedia.org/wiki/Phase-contrast_imaging

    In the field of transmission electron microscopy, phase-contrast imaging may be employed to image columns of individual atoms; a more common name is high-resolution transmission electron microscopy. It is the highest resolution imaging technique ever developed, and can allow for resolutions of less than one angstrom (less than 0.1 nanometres).

  6. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    Scientist using an optical microscope in a laboratory. The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present ...

  7. Phase-contrast X-ray imaging - Wikipedia

    en.wikipedia.org/wiki/Phase-contrast_X-ray_imaging

    X-ray absorption (left) and differential phase-contrast (right) image of an in-ear headphone obtained with a grating interferometer at 60kVp. Phase-contrast X-ray imaging or phase-sensitive X-ray imaging is a general term for different technical methods that use information concerning changes in the phase of an X-ray beam that passes through an object in order to create its images.

  8. Cell biology - Wikipedia

    en.wikipedia.org/wiki/Cell_biology

    The G1, G2, and S phase (DNA replication, damage and repair) are considered to be the interphase portion of the cycle, while the M phase is the cell division portion of the cycle. Mitosis is composed of many stages which include, prophase, metaphase, anaphase, telophase, and cytokinesis, respectively.

  9. Contrast transfer function - Wikipedia

    en.wikipedia.org/wiki/Contrast_transfer_function

    If we incorporate some assumptions about our sample, then an analytical expression can be found for both phase contrast and the phase contrast transfer function. As discussed earlier, when the electron wave passes through a sample, the electron beam interacts with the sample via scattering, and experiences a phase shift.

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