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Field of view is the area of the inspection captured on the camera’s imager. The size of the field of view and the size of the camera’s imager directly affect the image resolution (one determining factor in accuracy). Working distance is the distance between the back of the lens and the target object.
These lenses are often color coded for easier use. The least powerful lens is called the scanning objective lens, and is typically a 4× objective. The second lens is referred to as the small objective lens and is typically a 10× lens. The most powerful lens out of the three is referred to as the large objective lens and is typically 40–100×.
Lenses produced for 35 mm film cameras may mount well on the digital bodies, but the larger image circle of the 35 mm system lens allows unwanted light into the camera body, and the smaller size of the image sensor compared to 35 mm film format results in cropping of the image. This latter effect is known as field-of-view crop.
In wide-field multiphoton microscopy the high intensities are best achieved using an optically amplified pulsed laser source to attain a large field of view (~100 μm). [ 27 ] [ 28 ] [ 29 ] The image in this case is obtained as a single frame with a CCD camera without the need of scanning, making the technique particularly useful to visualize ...
The depth of field of an image produced at a given f-number is dependent on other parameters as well, including the focal length, the subject distance, and the format of the film or sensor used to capture the image. Depth of field can be described as depending on just angle of view, subject distance, and entrance pupil diameter (as in von Rohr ...
Memorial in Jena, Germany to Ernst Karl Abbe, who approximated the diffraction limit of a microscope as = , where d is the resolvable feature size, λ is the wavelength of light, n is the index of refraction of the medium being imaged in, and θ (depicted as α in the inscription) is the half-angle subtended by the optical objective lens (representing the numerical aperture).
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