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The solid phase now bears a dipeptide. This cycle is repeated to form the desired peptide chain. After all reactions are complete, the synthesised peptide is cleaved from the bead. The protecting groups for the amino groups mostly used in the peptide synthesis are 9-fluorenylmethyloxycarbonyl group and t-butyloxycarbonyl . A number of amino ...
The established method for the production of synthetic peptides in the lab is known as solid phase peptide synthesis (SPPS). [2] Pioneered by Robert Bruce Merrifield, [4] [5] SPPS allows the rapid assembly of a peptide chain through successive reactions of amino acid derivatives on a macroscopically insoluble solvent-swollen beaded resin support.
However, several small-size peptides and many large peptides and/or proteins are still unavailable by classical methods. Composition of a polyethylene glycol (PEG)-based resin. Recently, an efficient stepwise solid-phase synthesis of RANTES (24-91) was published. RANTES is a major HIV-suppressive factor produced by CD8+ T Cells. [18]
Merrifield Resin is a cross-linked polystyrene resin that carries a chloromethyl functional group. Merrifield resin is named after its inventor, Robert Bruce Merrifield (1984 winner of the Nobel Prize in Chemistry), and used in solid-phase synthesis. The material is typically available as white beads.
HBTU (Hexafluorophosphate Benzotriazole Tetramethyl Uronium) is a coupling reagent used in solid phase peptide synthesis. It was introduced in 1978 and shows resistance against racemization. [3] [4] It is used because of its mild activating properties. [5]
Combinatorial split-mix (split and pool) synthesis [12] [13] is based on the solid-phase synthesis developed by Merrifield. [14] If a combinatorial peptide library is synthesized using 20 amino acids (or other kinds of building blocks) the bead form solid support is divided into 20 equal portions.
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