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A stool test is a medical diagnostic technique that involves the collection and analysis of fecal matter. Microbial analysis (culturing), microscopy and chemical ...
Fecal occult blood testing (FOBT), as its name implies, aims to detect subtle blood loss in the gastrointestinal tract, anywhere from the mouth to the colon.Positive tests ("positive stool") may result from either upper gastrointestinal bleeding or lower gastrointestinal bleeding and warrant further investigation for peptic ulcers or a malignancy (such as colorectal cancer or gastric cancer).
Both square test areas in the upper area of the card show the intense blue color of a positive result. The lower two smaller circular areas on the orange stripe are analytical control reactions, positive on the left and negative on the right, that help assure that the card and developer bottle have been maintained in proper conditions and have not been damaged before the test is performed.
By growing and counting colonies of fecal coliform bacteria from a sample of water, the amount of bacteria originally present can be determined. Membrane filtration is the method of choice for the analysis of fecal coliforms in water. Samples to be tested are passed through a filter of a particular pore size (generally 0.45 micrometre). The ...
The presence of some coliforms indicate fecal contamination. The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate test. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate".
TSA plates support growth of many semifastidious bacteria, including some species of Brucella, Corynebacterium, Listeria, Neisseria, and Vibrio. Xylose-lysine-deoxycholate agar is used for the culture of stool samples and contains two indicators. It is formulated to inhibit Gram-positive bacteria, while the growth of Gram-negative bacilli is ...
An indole butyrate disc is used to differentiate between Neisseria gonorrhoeae (negative result) and Moraxella catarrhalis (positive result). This test involves a butyrate disk, which when smeared with a culture, will change color for a positive result after 5 minutes of incubation. A blue color is the result of a positive test. [29]
Indicator bacteria can be cultured on media which are specifically formulated to allow the growth of the species of interest and inhibit growth of other organisms. Typically, environmental water samples are filtered through membranes with small pore sizes and then the membrane is placed onto a selective agar.