Search results
Results from the WOW.Com Content Network
The diastase negative slide will show a magenta staining where glycogen is present within a section of tissue. The slide that has been treated with diastase will lack any positive PAS staining in those locations on the slide PAS staining is also used for staining cellulose. One example would be looking for implanted medical devices composed of ...
PAS diastase stain is also used to identify alpha-1 antitrypsin globules in hepatocytes, which is a characteristic finding of alpha-1 antitrypsin deficiency. [2] PAS diastase stain is also used in diagnosing Whipple’s disease , as the foamy macrophages that infiltrate the lamina propria of the small intestine in this disease possess PAS ...
Micrograph showing casts in a kidney biopsy using PAS stain: Hyaline casts are PAS-positive (dark pink/red, right of image). Myelomatous casts are PAS-negative (pale pink, left of image) Urinary casts are microscopic cylindrical structures produced by the kidney and present in the urine in certain disease states.
Ewing sarcoma is a small-blue-round-cell tumor that typically has a clear cytoplasm on H&E staining, due to glycogen. The presence of the glycogen can be demonstrated with positive PAS staining and negative PAS diastase staining. The characteristic immunostain is CD99, which diffusely marks the cell membrane.
Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. [2] While the first cases of IHC staining used fluorescent dyes (see immunofluorescence ), other non-fluorescent methods using enzymes such as peroxidase (see ...
Myeloma cast nephropathy, also referred to as light-chain cast nephropathy, is the formation of plugs (urinary casts) in the kidney tubules from free immunoglobulin light chains leading to kidney failure in the context of multiple myeloma.
At left is a micrograph of spleen with hyaline deposition (pink material at top of image) in association with inflammation (hyaloserositis), using H&E stain. At right is a micrograph of a kidney with arterial hyaline (hyaline arteriolosclerosis), using PAS stain.
Russell bodies were found to have positive reactions to PAS stain, CD 38 and CD 138 stains. Plasma cells that contain Russell bodies and are stained with H&E stain are found to be autofluorescent, while those without Russell bodies are not. [citation needed] Russell bodies tend to be found in places with chronic inflammation. [citation needed]