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Typical steam sterilization cycles are between 3 and 30 minutes at 121–134 °C (250–273 °F) at 100 kPa (15 psi), but adjustments may be made depending on the bioburden of the article being sterilized, its resistance to steam sterilization, the article's heat tolerance, and the required sterility assurance level. Following the completion of ...
An autoclave, which uses steam under pressure, is used in microbiology laboratories and similar environments for sterilization. Steam, especially dry (highly superheated) steam, may be used for antimicrobial cleaning even to the levels of sterilization. Steam is a non-toxic antimicrobial agent. [11] [12]
A vacuum pump sucks air or air/steam mixtures from the chamber. Superatmospheric cycles: Achieved with a vacuum pump. It starts with a vacuum followed by a steam pulse followed by a vacuum followed by a steam pulse. The number of pulses depends on the particular autoclave and cycle chosen. Subatmospheric cycles:
Vaporized hydrogen peroxide (trademarked VHP, [1] also known as hydrogen peroxide vapor, HPV) is a vapor form of hydrogen peroxide (H 2 O 2) with applications as a low-temperature antimicrobial vapor used to decontaminate enclosed and sealed areas such as laboratory workstations, isolation and pass-through rooms, [2] and even aircraft interiors.
Equipment used in aseptic processing of food and beverages must be sterilized before processing and remain sterile during processing. [1] When designing aseptic processing equipment there are six basic requirements to consider: the equipment must have the capability of being cleaned thoroughly, it must be able to be sterilized with steam, chemicals, or high-temperature water, sterilization ...
"F 0" is defined as the number of equivalent minutes of steam sterilization at temperature 121.1 °C (250 °F) delivered to a container or unit of product calculated using a z-value of 10 °C. The term F-value or "F Tref/z " is defined as the equivalent number of minutes to a certain reference temperature (T ref ) for a certain control ...
A waste autoclave is a form of solid waste treatment that uses heat, steam and pressure of an industrial autoclave in the processing of waste. Waste autoclaves process waste either in batches or in continuous-flow processes. In batch processes, saturated steam is pumped into the autoclave at temperatures around 160 °C, or 320 °F. [1]
Soil steam sterilization (soil steaming) is a farming technique that sterilizes soil with steam in open fields or greenhouses. Pests of plant cultures such as weeds, bacteria, fungi and viruses are killed through induced hot steam which causes vital cellular proteins to unfold. Biologically, the method is considered a partial disinfection.