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Scratch wound healing assay experiment of rhabdomyosarcoma, a cancer cell line. A wound healing assay is a laboratory technique used to study cell migration and cell–cell interaction. This is also called a scratch assay because it is done by making a scratch on a cell monolayer and capturing images at regular intervals by time lapse ...
The migration of cultured cells attached to a surface or in 3D is commonly studied using microscopy. [7] [8] [5] As cell movement is very slow, a few μm/minute, time-lapse microscopy videos are recorded of the migrating cells to speed up the movement.
Matrigel is the trade name for the solubilized basement membrane matrix secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells produced by Corning Life Sciences. . Matrigel resembles the laminin/collagen IV-rich basement membrane extracellular environment found in many tissues and is used by cell biologists as a substrate (basement membrane matrix) for culturi
Collective cell migration describes the movements of group of cells and the emergence of collective behavior from cell-environment interactions and cell-cell communication. Collective cell migration is an essential process in the lives of multicellular organisms , e.g. embryonic development , wound healing and cancer spreading ( metastasis ). [ 1 ]
The most common technique to add inserts to desired sequences is the use of homologous recombination. [5] This technique has a specific requirement where the insert can only be added after it has been introduced to the nucleus of the cell, which can be added to the genome mostly during the G2 and S phases in the cell cycle. [6]
NFL fans are gearing up for one of the best holiday traditions in sports. The league is set to kick off its playoff push with three Thanksgiving games, featuring several teams vying for playoff spots.
Whether you’re preparing a spiral-cut ham for 10, or serving up an appetizer of prosciutto and jamón serrano on a charcuterie board, ham is a versatile meat that often makes an appearance as ...
For proteins, SDS-PAGE is usually the first choice as an assay of purity due to its reliability and ease. The presence of SDS and the denaturing step make proteins separate, approximately based on size, but aberrant migration of some proteins may occur. Different proteins may also stain differently, which interferes with quantification by staining.
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