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2. Reversed-phase chromatography - Wikipedia

   en.wikipedia.org/wiki/Reversed-phase_chromatography

   Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...

3. Aqueous normal-phase chromatography - Wikipedia

   en.wikipedia.org/wiki/Aqueous_normal-phase...

   In reversed phase the opposite is true; the stationary phase is nonpolar and the mobile phase is polar. Typical stationary phases for normal-phase chromatography are silica or organic moieties with cyano and amino functional groups. For reversed phase, alkyl hydrocarbons are the preferred stationary phase; octadecyl (C18) is the most common ...

4. Chromatography - Wikipedia

   en.wikipedia.org/wiki/Chromatography

   The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column. In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated. The mobile phase ...

5. High-performance liquid chromatography - Wikipedia

   en.wikipedia.org/wiki/High-performance_liquid...

   The most common mode of liquid chromatography is reversed phase, whereby the mobile phases used, include any miscible combination of water or buffers with various organic solvents (the most common are acetonitrile and methanol). Some HPLC techniques use water-free mobile phases (see normal-phase chromatography below).

6. Hydrophilic interaction chromatography - Wikipedia

   en.wikipedia.org/wiki/Hydrophilic_interaction...

   Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase ...

7. Thin-layer chromatography - Wikipedia

   en.wikipedia.org/wiki/Thin-layer_chromatography

   If the stationary phase is non-polar, like C18-functionalized silica plates, it is called reverse-phase TLC. In this case, non-polar compounds move less and polar compounds move more. [citation needed] The solvent mixture will also be much more polar than in normal-phase TLC. [11]

8. Column chromatography - Wikipedia

   en.wikipedia.org/wiki/Column_chromatography

   The most common stationary phase for column chromatography is silica gel, the next most common being alumina. Cellulose powder has often been used in the past. A wide range of stationary phases are available in order to perform ion exchange chromatography, reversed-phase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA

9. Micellar liquid chromatography - Wikipedia

   en.wikipedia.org/wiki/Micellar_liquid_chromatography

   Reverse phase high-performance liquid chromatography (RP-HPLC) involves a non-polar stationary phase, often a hydrocarbon chain, and a polar mobile or liquid phase. The mobile phase generally consists of an aqueous portion with an organic addition, such as methanol or acetonitrile .