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TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.. Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not ...
Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol . DTT is redox reagent also known as Cleland's reagent , after W. Wallace Cleland . [ 2 ]
Dithiobutylamine (DTBA) is a reducing agent intended as an alternative for DTT in biochemical uses. It was designed to be easily synthesized in non-racemic form, to have a lower pKa (allowing more effective reduction at neutral pH), and to have a low disulfide E°′ reduction potential. [1]
2-Mercaptoethanol is often used interchangeably with dithiothreitol (DTT) or the odorless tris(2-carboxyethyl)phosphine (TCEP) in biological applications. Although 2-mercaptoethanol has a higher volatility than DTT, it is more stable: 2-mercaptoethanol's half-life is more than 100 hours at pH 6.5 and 4 hours at pH 8.5; DTT's half-life is 40 ...
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A Canadian woman was arrested after trying to smuggle over 20 pounds of methamphetamine through a New Zealand airport, authorities said. The illicit drugs were disguised as Christmas presents, New ...
The Annual Review of Biomedical Engineering was first published in 1999 by the nonprofit publisher Annual Reviews. [4] The inaugural editor was Martin L. Yarmush; [5] Yarmush remained editor until 2021, at which point he was co-editor along with Mehmet Toner. [6] Though it began with a physical edition, it is now only published electronically. [7]
(Reuters) -Four men were convicted in Spain on Sunday in connection with the homophobic murder of a 24-year-old nursing assistant that sparked protests in cities across Spain and abroad.