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At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA is 0.020 (μg/mL) −1 cm −1, for single-stranded DNA it is 0.027 (μg/mL) −1 cm −1, for single-stranded RNA it is 0.025 (μg/mL) −1 cm −1 and for short single-stranded oligonucleotides it is dependent on the length and base composition.
In biochemistry, the molar absorption coefficient of a protein at 280 nm depends almost exclusively on the number of aromatic residues, particularly tryptophan, and can be predicted from the sequence of amino acids. [6] Similarly, the molar absorption coefficient of nucleic acids at 260 nm can be predicted given the nucleotide sequence.
nM: 5 nM: inhaled osmium tetroxide is immediately dangerous to life or health (1 mg Os/m 3) [13] 10 −8: 10 −7: 101 nM: hydronium and hydroxide ions in pure water at 25 °C (pK W = 13.99) [14] 10 −6: μM: 10 −5: 10 −4: 180–480 μM: normal range for uric acid in blood [10] 570 μM
To create the solution, 11.6 g NaCl is placed in a volumetric flask, dissolved in some water, then followed by the addition of more water until the total volume reaches 100 mL. The density of water is approximately 1000 g/L and its molar mass is 18.02 g/mol (or 1/18.02 = 0.055 mol/g). Therefore, the molar concentration of water is
Add 5.0 mL of Coomassie Blue to each tube and mix by vortex, or inversion. Adjust the spectrophotometer to a wavelength of 595 nm, using the tube which contains no protein (blank). Wait 5 minutes and read each of the standards and each of the samples at 595 nm wavelength. Plot the absorbance of the standards vs. their concentration.
Fluorescein aqueous solutions, diluted from 10,000 to 1 parts-per-million in intervals of 10 fold dilution. At 1 ppm the solution is a very pale yellow. As the concentration increases the colour becomes a more vibrant yellow, then orange, with the final 10,000 ppm a deep red colour.
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1 Nm 3 of any gas (measured at 0 °C and 1 atmosphere of absolute pressure) equals 37.326 scf of that gas (measured at 60 °F and 1 atmosphere of absolute pressure). 1 kmol of any ideal gas equals 22.414 Nm 3 of that gas at 0 °C and 1 atmosphere of absolute pressure ... and 1 lbmol of any ideal gas equals 379.482 scf of that gas at 60 °F and ...