Search results
Results from the WOW.Com Content Network
Golden Gate assembly involves digesting DNA sequences containing a type IIS restriction enzyme cut site and ligating them together. Golden Gate Cloning or Golden Gate assembly [1] is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase. [2]
Cassette mutagenesis via Golden Gate Assembly. Cassette mutagenesis is a type of site-directed mutagenesis that uses a short, double-stranded oligonucleotide sequence (gene cassette) to replace a fragment of target DNA. It uses complementary restriction enzyme digest ends on the target DNA and gene cassette to achieve specificity.
The Golden Braid assembly standard also builds on the first tier of Golden Gate assembly and assembles further tiers via a pairwise protocol. Four tier one destination vectors (assembled via Golden Gate assembly) are assembled into two tier 2 destination vectors, which are then used as tier 3 entry vectors for the tier 3 destination vector.
Golden Gate assembly: Software used: Serif Affinity Designer (Mar 31 2020) Conversion program: PDFlib+PDI 9.1.2p1-i (OS X/macOS) Encrypted: no: Page size: 468 x 720 pts:
Get AOL Mail for FREE! Manage your email like never before with travel, photo & document views. Personalize your inbox with themes & tabs. You've Got Mail!
You are free: to share – to copy, distribute and transmit the work; to remix – to adapt the work; Under the following conditions: attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made.
Upgrade to a faster, more secure version of a supported browser. It's free and it only takes a few moments:
The car was damaged beyond repair. 356/2-061 This coupe was configured as a SL in Zuffenhausen in 1951 with chassis number 3004/A and was ordered by D'Ieteren Belgium on September 19, 1951. [ 11 ] After appearing in the 1952 Brussels Auto Show it was sold to Max Thirion and raced extensively by his daughter Gilberte Thirion.