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Bloxsom developed the air lock device based on his assumptions about the role of uterine contractions in establishing effective breathing at birth. The air lock was a sealed steel cylinder that delivered warmed and humidified 60% oxygen to newly born babies. The device has been compared to an iron lung, but it did not utilize negative pressure.
[1] This table displays the steps involved in using a GasPak to create a completely anaerobic environment for incubating an inoculated agar plate. After inoculating the agar plate(s) with bacteria under aseptic conditions, the agar plates are placed in an anaerobic jar that contains components, like the catalyst chamber, that will help facilitate the reaction to eliminate free oxygen and ...
The air in the incubator was kept at 37 degrees Celsius, the same temperature as the human body, and the incubator maintained the atmospheric carbon dioxide and nitrogen levels necessary to promote cell growth. At this time, incubators also began to be used in genetic engineering. Scientists could create biologically essential proteins, such as ...
It is very important to ensure that all of the trapped air is removed from the autoclave before activation, as trapped air is a very poor medium for achieving sterility. Steam at 134 °C (273 °F) can achieve a desired level of sterility in three minutes, while achieving the same level of sterility in hot air requires two hours at 160 °C (320 ...
A laminar flow cabinet blows unfiltered exhaust air towards the worker and is not safe for work with pathogenic agents, [2]: 13 [3] while a fume hood maintains negative pressure with constant exhaust to protect the user, but does not protect the work materials from contamination by the surrounding environment.
A resuscitator is a device using positive pressure to inflate the lungs of an unconscious person who is not breathing, in order to keep them oxygenated and alive. [citation needed] There are three basic types: a manual version (also known as a bag valve mask) consisting of a mask and a large hand-squeezed plastic bulb using ambient air, or with supplemental oxygen from a high-pressure tank.
The gas in a glovebox is pumped through a series of treatment devices which remove solvents, water and oxygen from the gas. Copper metal (or some other finely divided metal) is commonly used to remove oxygen, this oxygen removing column is normally regenerated by passing a hydrogen/nitrogen mixture through it while it is heated: the water formed is passed out of the box with the excess ...
The culture media to be tested is then added to the larger tube and sterilized, which also eliminates the initial air gap produced when the tube is inserted upside down. The culture media typically contains a single substance to be tested with the organism, such as to determine whether an organism can ferment a particular carbohydrate.