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Less than 20 tonnes of CsCl is produced annually worldwide, mostly from a caesium-bearing mineral pollucite. [7] Caesium chloride is widely used in isopycnic centrifugation for separating various types of DNA. It is a reagent in analytical chemistry, where it is used to
Historically a cesium chloride (CsCl) solution was often used, but more commonly used density gradients are sucrose or Percoll.This application requires a solution with high density and yet relatively low viscosity, and CsCl suits it because of its high solubility in water, high density owing to the large mass of Cs, as well as low viscosity and high stability of CsCl solutions.
Differential centrifugation is the simplest method of fractionation by centrifugation, [9] commonly used to separate organelles and membranes found in cells. Organelles generally differ from each other in density and in size, making the use of differential centrifugation, and centrifugation in general, possible.
Isopycnic centrifugation refers to a method wherein a density gradient is either pre-formed or forms during high speed centrifugation. After this gradient is formed particles move within the gradient to the position having a density matching their own (this is in fact an incorrect description of the exact physical process but does describe the ...
The method is distinguished from zone-sedimentation in that a stabilizing density gradient is self-generated during centrifugation, through the use of a higher density (than the sample) bulk "binary solvent", containing both a solvent (i.e. H 2 O), and a second component (small molecules, i.e. CsCl) that will sediment to form a stabilizing ...
Differential centrifugation. In biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate.
Laboratory centrifuge. There are various types of centrifugation: Differential centrifugation, often used to separate certain organelles from whole cells for further analysis of specific parts of cells; Isopycnic centrifugation, often used to isolate nucleic acids such as DNA
Bisbenzimide tends to bind to adenine–thymine-rich regions of DNA and can decrease its density.Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.