Search results
Results from the WOW.Com Content Network
Ames test procedure. The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. More formally, it is a biological assay to assess the mutagenic potential of chemical compounds. [1]
From the pool of G 1 individuals, a heterozygous male is crossed to a female carrying the mutant allele (a). If the G 2 progeny are infertile or non-viable, they can be recovered again from the G 1 male. Figure 4: Deletion Screens.In this screen, ENU-treated males are crossed to females homozygous for a deletion of the region of interest. The ...
In other words, there is a feasible recombination possibility between the point mutant and del-2 in which a length of DNA could be made that contained neither the point mutation, nor the deletion, indicating that the mutations in these two strains cannot be in the same region. Note that not all crossovers between the point mutant and del-2 will ...
Types of mutations that can be introduced by random, site-directed, combinatorial, or insertional mutagenesis. In molecular biology, mutagenesis is an important laboratory technique whereby DNA mutations are deliberately engineered to produce libraries of mutant genes, proteins, strains of bacteria, or other genetically modified organisms.
The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...
It is a yellow solid [1] with planar molecules [2] that exhibits ferroelectric properties. [3] The compound is a weak acid: one or both hydroxyls can lose a proton to yield the anions C 6 H 3 O − 4 (pK a1 = 2.95) and C 6 H 2 O 2− 4 (pK a2 = 4.87), respectively. The latter forms a variety of metal complexes, functioning as a binucleating ...
A clastogen is a mutagenic agent that disturbs normal DNA related processes or directly causes DNA strand breakages, thus causing the deletion, insertion, or rearrangement of entire chromosome sections. [1] These processes are a form of mutagenesis which if left unrepaired, or improperly repaired, can lead to cancer. [1]
Particles contain 7.5 kb DNA fragments. Production of VSH-1 is stimulated by the DNA-damaging agent mitomycin C and by some antibiotics. It is also associated with detectable cell lysis, indicating that a substantial fraction of the culture may be producing VSH-1. [20]