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  2. Langmuir adsorption model - Wikipedia

    en.wikipedia.org/wiki/Langmuir_adsorption_model

    The concentration of sites is given by dividing the total number of sites (S 0) covering the whole surface by the area of the adsorbent (a): [ S 0 ] = S 0 / a . {\displaystyle [S_{0}]=S_{0}/a.} We can then calculate the concentration of all sites by summing the concentration of free sites [ S ] and occupied sites:

  3. Variable pathlength cell - Wikipedia

    en.wikipedia.org/wiki/Variable_pathlength_cell

    Variable pathlength absorption spectroscopy uses a determined slope to calculate concentration. As stated above this is a product of the molar absorptivity and the concentration. Since the actual absorbance value is taken at many data points at equal intervals, background subtraction is generally unnecessary.

  4. Absorbance - Wikipedia

    en.wikipedia.org/wiki/Absorbance

    Absorbance is defined as "the logarithm of the ratio of incident to transmitted radiant power through a sample (excluding the effects on cell walls)". [1] Alternatively, for samples which scatter light, absorbance may be defined as "the negative logarithm of one minus absorptance, as measured on a uniform sample". [2]

  5. Beer–Lambert law - Wikipedia

    en.wikipedia.org/wiki/Beer–Lambert_law

    The amount concentration c is then given by = (). For a more complicated example, consider a mixture in solution containing two species at amount concentrations c 1 and c 2 . The decadic attenuation coefficient at any wavelength λ is, given by μ 10 ( λ ) = ε 1 ( λ ) c 1 + ε 2 ( λ ) c 2 . {\displaystyle \mu _{10}(\lambda )=\varepsilon _{1 ...

  6. Nucleic acid quantitation - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_quantitation

    In essence, the Beer Lambert Law makes it possible to relate the amount of light absorbed to the concentration of the absorbing molecule. The following absorbance units to nucleic acid concentration conversion factors are used to convert OD to concentration of unknown nucleic acid samples: [5] A260 dsDNA = 50 μg/mL A260 ssDNA = 33 μg/mL

  7. Molar absorption coefficient - Wikipedia

    en.wikipedia.org/wiki/Molar_absorption_coefficient

    The absorbance of a material that has only one absorbing species also depends on the pathlength and the concentration of the species, according to the Beer–Lambert law =, where ε is the molar absorption coefficient of that material; c is the molar concentration of those species; ℓ is the path length.

  8. Cross section (physics) - Wikipedia

    en.wikipedia.org/wiki/Cross_section_(physics)

    The total cross section is related to the absorbance of the light intensity through the Beer–Lambert law, which says that absorbance is proportional to concentration: A λ = Clσ, where A λ is the absorbance at a given wavelength λ, C is the concentration as a number density, and l is the path length.

  9. Cavity ring-down spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Cavity_ring-down_spectroscopy

    The technique is also known as cavity ring-down laser absorption ... calculate the concentration of ... of a rate constant. Wide range of use for a given set of ...