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Because the appearance of microbial colonies changes as they grow, colonial morphology is examined at a specific time after the plate is inoculated. Usually, the plate is read at 18–24 hours post-inoculation, [1]: 163 but times may differ for slower-growing organisms like fungi.
An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
When some otherwise non-hemolytic bacteria, such as strains of the cholera-causing bacteria Vibrio cholerae, are plated on blood agar, no clearings are observed surrounding isolated colonies, but the blood surrounding larger areas of growth turns green. This process, called hemodigestion, is caused by the metabolic by-products of the bacteria ...
A Petri dish with bacterial colonies on an agar-based growth medium Axenic cell culture of the plant Physcomitrella patens on an agarplate in a Petri dish. Petri dishes are widely used in biology to cultivate microorganisms such as bacteria, yeasts, and molds. It is most suited for organisms that thrive on a solid or semisolid surface.
Colony-forming units are used to quantify results in many microbiological plating and counting methods, including: The pour plate method wherein the sample is suspended in a Petri dish using molten agar cooled to approximately 40–45 °C (just above the point of solidification to minimize heat-induced cell death).
A bacterial lawn used in antibiotic resistance testing. Bacterial lawn is a term used by microbiologists to describe the appearance of bacterial colonies when all the individual colonies on a Petri dish or agar plate merge to form a field or mat of bacteria. Bacterial lawns find use in screens for antibiotic resistance and bacteriophage titering.