Search results
Results from the WOW.Com Content Network
An oligomer formed by one of proteins from Type VI secretion system in Burkholderia pseudomallei. The T6SS is thought to resemble an inverted phage extending outward from the bacterial cell surface. It consists of 14 proteins that assemble into three sub-complexes: a phage tail-like tubule, a phage baseplate-like structure, and cell-envelope ...
However, some tailed bacteriophage genomes can vary quite significantly in nucleotide sequence, even among the same genus. Due to their characteristic structure and possession of potentially homologous genes, it is believed these bacteriophages possess a common origin.
Structural model at atomic resolution of bacteriophage T4 [1] The structure of a typical myovirus bacteriophage Anatomy and infection cycle of bacteriophage T4. A bacteriophage (/ b æ k ˈ t ɪər i oʊ f eɪ dʒ /), also known informally as a phage (/ ˈ f eɪ dʒ /), is a virus that infects and replicates within bacteria and archaea.
Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions.In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively.
During 1962-1964 phage T4 researchers provided an opportunity to study the function of virtually all of the genes that are essential for growth of the phage under laboratory conditions. [43] [44] These studies were facilitated by the discovery of two classes of conditional lethal mutants. One class of such mutants is known as amber mutants. [45]
The RM system was first discovered by Salvatore Luria and Mary Human in 1952 and 1953. [1] [2] They found that a bacteriophage growing within an infected bacterium could be modified, so that upon their release and re-infection of a related bacterium the bacteriophage's growth is restricted (inhibited; also described by Luria in his autobiography on pages 45 and 99 in 1984). [3]
A prophage is a bacteriophage (often shortened to "phage") genome that is integrated into the circular bacterial chromosome or exists as an extrachromosomal plasmid within the bacterial cell. [1] Integration of prophages into the bacterial host is the characteristic step of the lysogenic cycle of temperate phages.
P1 is a temperate bacteriophage that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle the phage genome exists as a plasmid in the bacterium [1] unlike other phages (e.g. the lambda phage) that integrate into the host DNA.