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Equipment used in aseptic processing of food and beverages must be sterilized before processing and remain sterile during processing. [1] When designing aseptic processing equipment there are six basic requirements to consider: the equipment must have the capability of being cleaned thoroughly, it must be able to be sterilized with steam, chemicals, or high-temperature water, sterilization ...
The goal of asepsis is to eliminate infection, not to achieve sterility. [1] Ideally, a surgical field is sterile, meaning it is free of all biological contaminants (e.g. fungi, bacteria, viruses), not just those that can cause disease, putrefaction, or fermentation. [1] Even in an aseptic state, a condition of sterile inflammation may develop.
Microorganisms growing on an agar plate. Sterilization (British English: sterilisation) refers to any process that removes, kills, or deactivates all forms of life (particularly microorganisms such as fungi, bacteria, spores, and unicellular eukaryotic organisms) and other biological agents (such as prions or viruses) present in fluid or on a specific surface or object. [1]
Sterile or sterility may refer to: Asepsis, a state of being free from biological contaminants; Sterile (archaeology), a sediment deposit which contains no evidence of human activity; Sterilization (microbiology), any process that eliminates or kills all forms of life or removes them from an item or a field
Introducing a potentially non-sterile needle into the bioprocessing equipment violates good aseptic technique. With a cannula based aseptic sampling device [4] the needle and septa are combined into a hermetically sealed aseptic sampling device.
Sterile dental instruments from hospital central supply (barcoded label indicating sterilization date, expiry date and contents). The central sterile services department (CSSD), also called sterile processing department (SPD), sterile processing, central supply department (CSD), or central supply, is an integrated place in hospitals and other health care facilities that performs sterilization ...
Aseptic technique is then applied to the withdrawn inoculation needle. Plate culture. Inoculation of a plate culture is done through the streaking technique to make a streak plate. [1] [2] [4] After lifting the lid so that it hovers above the sterile agar plate, the inoculation needle will be streaked across the plate in controlled directions. [1]
Axenic cultures of microorganisms are typically prepared by subculture of an existing mixed culture. This may involve use of a dilution series, in which a culture is successively diluted to the point where subsamples of it contain only a few individual organisms, ideally only a single individual (in the case of an asexual species).