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Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
Schematic diagram of the arrangement of optical components in a typical Spectrofluorometer. A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample.
Fluorometer designed to measure chlorophyll fluorescence in plants. A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1]
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Block diagram of an analytical instrument showing the stimulus and measurement of response. ... X-ray fluorescence spectroscopy, infrared spectroscopy, ...
Table-top spectrophotometer Beckman IR-1 Spectrophotometer, c. 1941 Beckman Model DB Spectrophotometer (a double beam model), 1960 Hand-held spectrophotometer used in graphic industry [1] Spectrophotometry is a branch of electromagnetic spectroscopy concerned with the quantitative measurement of the reflection or transmission properties of a ...
Jablonski diagram including vibrational levels for absorbance, non-radiative decay, and fluorescence. When a molecule absorbs a photon, the photon energy is converted and increases the molecule's internal energy level. Likewise, when an excited molecule releases energy, it can do so in the form of a photon.
Diagram of the Stokes shift between absorption and emission light spectra. Stokes shift is the difference (in energy, wavenumber or frequency units) between positions of the band maxima of the absorption and emission spectra (fluorescence and Raman being two examples) of the same electronic transition. [1]