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Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]
So, for example, if a route is 20 kilometres (12 mi) with 1600 metres of climb (as is the case on leg 1 of the Bob Graham Round, Keswick to Threlkeld), the equivalent flat distance of this route is 20+(1.6×8)=32.8 kilometres (20.4 mi). Assuming an individual can maintain a speed on the flat of 5 km/h, the route will take 6 hours and 34 minutes.
There are two common methods in which to construct a DNA molecular-weight size marker. [3] One such method employs the technique of partial ligation. [3] DNA ligation is the process by which linear DNA pieces are connected to each other via covalent bonds; more specifically, these bonds are phosphodiester bonds. [4]
Primer walking is a method to determine the sequence of DNA up to the 1.3–7.0 kb range whereas chromosome walking is used to produce the clones of already known sequences of the gene. [2] Too long fragments cannot be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into ...
Sequencing technologies vary in the length of reads produced. Reads of length 20-40 base pairs (bp) are referred to as ultra-short. [2] Typical sequencers produce read lengths in the range of 100-500 bp. [3] However, Pacific Biosciences platforms produce read lengths of approximately 1500 bp. [4] Read length is a factor which can affect the results of biological studies. [5]
After crunching the data, the researchers discovered that people who logged 75 minutes of moderate-intensity exercise (which includes brisk walking) per week had a 23 percent lower risk of early ...
Kira Jones, personal trainer and founder of Cacti Wellness Collective, tells Yahoo Life that because extreme walks can take “over three hours and likely burn around 1,000 calories,” doing so ...
The level of unique gene expression is represented by the count of transcripts present per million molecules, similar to SAGE output. A significant advantage is the larger library size compared with SAGE. An MPSS library typically holds 1 million signature tags, which is roughly 20 times the size of a SAGE library.