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A serial dilution is the step-wise dilution of a substance in solution, either by using a constant dilution factor, or by using a variable factor between dilutions. If the dilution factor at each step is constant, this results in a geometric progression of the concentration in a logarithmic fashion.
Fluorescein aqueous solutions, diluted from 10 000 to 1 part per million in intervals of ten-fold dilution. At 10 000 ppm the solution is a deep red colour. As the concentration decreases the colour becomes orange, then a vibrant yellow, with the final 1 ppm sample a very pale yellow.
The titer corresponds to the highest dilution factor that still yields a positive reading. [2] For example, positive readings in the first 8 serial, twofold dilutions translate into a titer of 1:256 (i.e., 2 −8). Titres are sometimes expressed by the denominator only, for example 1:256 is written 256. [3]
The diluted material must be thoroughly mixed to achieve the true dilution. For example, in a solution with a 1:5 dilution ratio, entails combining 1 unit volume of solute (the material to be diluted) with 5 unit volumes of the solvent to give 6 total units of total volume. In photographic development, dilutions are normally given in a '1+x ...
Term Description Examples Autocracy: Autocracy is a system of government in which supreme power (social and political) is concentrated in the hands of one person or polity, whose decisions are subject to neither external legal restraints nor regularized mechanisms of popular control (except perhaps for the implicit threat of a coup d'état or mass insurrection).
Typically, ten-fold dilutions are used, and the dilution series is plated in replicates of 2 or 3 over the chosen range of dilutions. Often 100 μL are plated but also larger amounts up to 1 mL are used. Higher plating volumes increase drying times but often do not result in higher accuracy, since additional dilution steps may be needed. [5]
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[contradictory] For example, the number 4 000 000 has a logarithm (in base 10) of 6.602; its order of magnitude is 6. When truncating, a number of this order of magnitude is between 10 6 and 10 7 . In a similar example, with the phrase "seven-figure income", the order of magnitude is the number of figures minus one, so it is very easily ...