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Schematic of cell adhesion. Cell adhesion is the process by which cells interact and attach to neighbouring cells through specialised molecules of the cell surface. This process can occur either through direct contact between cell surfaces such as cell junctions or indirect interaction, where cells attach to surrounding extracellular matrix, a gel-like structure containing molecules released ...
Adherent cells require a surface, such as tissue culture plastic or microcarrier, which may be coated with extracellular matrix (such as collagen and laminin) components to increase adhesion properties and provide other signals needed for growth and differentiation. Most cells derived from solid tissues are adherent.
The best-characterized ligand for the three selectins is P-selectin glycoprotein ligand-1 , which is a mucin-type glycoprotein expressed on all white blood cells. Selectins have been implicated in several roles but they are especially important in the immune system by helping white blood cell homing and trafficking. [20]
Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur. [1] Most vertebrate derived cells (with the exception of hematopoietic cells) can be cultured and require a 2 dimensional monolayer that to facilitate cell adhesion and spreading. [2]
In cell culture biology, confluence refers to the percentage of the surface of a culture dish that is covered by adherent cells.For example, 50 percent confluence means roughly half of the surface is covered, while 100 percent confluence means the surface is completely covered by the cells, and no more room is left for the cells to grow as a monolayer. [1]
Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to cell culture, trypsin breaks down the proteins that enable the cells to adhere to the vessel.
Oligodendrocytes are a type of glial cell, non-neuronal cells in the central nervous system.They arise during development from oligodendrocyte precursor cells (OPCs), [8] which can be identified by their expression of a number of antigens, including the ganglioside GD3, [9] [10] [11] the NG2 chondroitin sulfate proteoglycan, and the platelet-derived growth factor-alpha receptor subunit (PDGF ...
CHO cells in suspension. A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. The ...