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The Kinyoun method or Kinyoun stain (cold method), developed by Joseph J. Kinyoun, is a procedure used to stain acid-fast species of the bacterial genus Mycobacterium. [1] It is a variation of a method developed by Robert Koch in 1882. Certain species of bacteria have a waxy lipid called mycolic acid, in their cell walls which allow them to be ...
Joseph James "Joe" Kinyoun was born November 25, 1860, in East Bend, North Carolina, the oldest of five children born to Elizabeth Ann Conrad and John Hendricks Kinyoun.. His family settled in Post Oak, Missouri in 1866 after his house burned down during the Civil W
Ziehl and Neelsen's modifications together have developed the Ziehl–Neelsen stain. Another acid-fast stain was developed by Joseph Kinyoun by using the Ziehl–Neelsen staining technique but removing the heating step from the procedure. This new stain from Kinyoun was named the Kinyoun stain. [21]
Kinyoun stain; For color blind people (or in backgrounds where detecting red bacteria is difficult), Victoria blue can be substituted for carbol fuchsin and picric acid can be used as the counter stain instead of methylene blue, and the rest of the Kinyoun technique can be used. [6] Various bacterial spore staining techniques using Kenyon e.g.
[13] [57] The most common acid-fast staining techniques are the Ziehl–Neelsen stain [59] and the Kinyoun stain, which dye acid-fast bacilli a bright red that stands out against a blue background. [60] Auramine-rhodamine staining [61] and fluorescence microscopy [62] are also used.
We love to cook our favorite casseroles and soups, but staining can inevitably occur and bits of food will become stuck. Avoid using any type of metal tool or spatula to scrape the surface. Metal ...
Hospice Advantage of Pelham. Hospice Advantage, LLC purchased Hospice Care of Pelham from HC Healthcare, Inc. on April 1, 2012. Two years later, on April 14, 2014, an initial Hospice Accreditation survey with the Community Health Accreditation Program (CHAP) was held.
Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint (CAS) is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes.