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The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...
The second method of histology processing is called frozen section processing. This is a highly technical scientific method performed by a trained histoscientist. In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat. The thin frozen sections are mounted on ...
As the CCPDMA surgery is frequently performed using frozen section pathology, immediate reporting of positive surgical margin is made, and the tumor can be completely removed in the same day. Traditional pathology processing is called "bread loafing", and only allows for the partial examination of the surgical margin.
Toluidine blue is also commonly used to stain frozen sections (rapid microscopic analysis of a specimen). Because time is of the essence for a frozen section, toluidine blue allows for the frozen section to be stained and reviewed in 10 to 20 seconds. [4] The other staining method for frozen sections (rapid H&E) takes approximately 60 to 90 ...
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature compound (OCT compound) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections ...
The SEE-FIM protocol is a pathology dissection protocol for Sectioning and Extensively Examining the Fimbria (SEE-FIM). This protocol is intended to provide for the optimal microscopic examination of the distal fallopian tube (fimbria) to identify either cancerous or precancerous conditions in this organ.
Cryostats are used in medicine to cut histological slides. They are usually used in a process called frozen section histology (see Frozen section procedure). The cryostat is essentially an ultrafine "deli-slicer", called a microtome, placed in a freezer. The cryostat is usually a stationary upright freezer, with an external wheel for rotating ...
Modern frozen section method. Frozen section histology does not give the added margin of safety by the cytotoxic Mohs paste, [14] originally used by Mohs. This paste might have destroyed any residual cancer cells not detected by the pathologist. Missing epidermal margins. Ideally, the Mohs section should include 100% of the epidermal margin ...