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Electrical cell fusion is an essential step in some of the most innovative methods in modern biology. This method begins when two cells are brought into contact by dielectrophoresis . Dielectrophoresis uses a high frequency alternating current, unlike electrophoresis in which a direct current is applied.
Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same phase. Cell synchrony is a vital process in the study of cells progressing through the cell cycle as it allows population-wide data to be collected rather than relying solely on single-cell experiments.
Heterokaryon in fungal mitosis. A heterokaryon is a multinucleate cell that contains genetically different nuclei. Heterokaryotic and heterokaryosis are derived terms. This is a special type of syncytium.
Cell fusion is the formation of a hybrid cell from two separate cells. [1] [2] There are three major actions taken in both virus–cell fusion and cell–cell fusion: the dehydration of polar head groups, the promotion of a hemifusion stalk, and the opening and expansion of pores between fusing cells. [3]
Cell–cell fusogens are proteins that promote plasma membrane fusion among different cells. To be considered a fusogen, it must be required for fusion, fuse unfamiliar membranes, and be present on the fusing membrane when need be. These cells include but are not limited too: gametes, trophoblasts, epithelial, and other developmental cells.
A classic example of a syncytium is the formation of skeletal muscle.Large skeletal muscle fibers form by the fusion of thousands of individual muscle cells. The multinucleated arrangement is important in pathologic states such as myopathy, where focal necrosis (death) of a portion of a skeletal muscle fiber does not result in necrosis of the adjacent sections of that same skeletal muscle ...
Karyogamy in the context of cell fusion. 1-haploid cells, 2-cell fusion, 3-single cell with two pronuclei, 4-fusing pronuclei (karyogamy), 5-diploid cell Karyogamy is the final step in the process of fusing together two haploid eukaryotic cells, and refers specifically to the fusion of the two nuclei .
CID has been used for a number of applications in biomedical research. In most applications each dimerizing protein is expressed as part of a fusion construct with other proteins of interest. Adding the chemical dimerizing agent brings both constructs into proximity with each other and induces interactions between the proteins of interest.