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Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line. After several transmissions, MEFs will senesce and finally die off.
EMAGE (e-Mouse Atlas of Gene Expression [note 1]) is an online biological database of gene expression data in the developing mouse (Mus musculus) embryo. [1] [2] [3] The data held in EMAGE is spatially annotated to a framework of 3D mouse embryo models produced by EMAP (e-Mouse Atlas Project).
The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 × 10 5 cells per 20 cm 2 dish (the second "3") continuously. [2] The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3 ...
A typical frog embryo, incubated at 18 °C, is an early stage neurula by 50 hours post-fertilization and a late stage neurula by 67 hours. [3] The mouse embryo begins neurulation on day 7.5 of gestation and remains in the neurula stage until day 9. [4]
The "fate" of each cell or group of cells is mapped onto the embryo, showing which parts of the embryo will develop into which tissue. When carried out at single-cell resolution, this process is called cell lineage tracing. It is also used to trace the development of tumors. Fate mapping and cell lineage are similar methods for tracing the ...
Diagram of stages of embryo development to a larval and adult stage. In developmental biology, animal embryonic development, also known as animal embryogenesis, is the developmental stage of an animal embryo. Embryonic development starts with the fertilization of an egg cell (ovum) by a sperm cell (spermatozoon). [1]
The earliest specification in mouse embryos occurs between trophoblast and inner cell mass cells in the outer polar cells and the inner apolar cells respectively. These two groups become specified at the eight-cell stage during compaction, but do not become determined until they reach the 64-cell stage.
Primary Concern: It is easy to be too detailed by including many different needs in a single diagram. This should be avoided. It is better to draw multiple diagrams, one for each viewpoint, than to draw a 'mega diagram' that is extremely rich in content. Remember this: when building houses, the architect delivers many different diagrams.