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Phosphoenolpyruvate (2-phosphoenolpyruvate, PEP) is the carboxylic acid derived from the enol of pyruvate and phosphate. It exists as an anion. PEP is an important intermediate in biochemistry. It has the highest-energy phosphate bond found (−61.9 kJ/mol) in organisms, and is involved in glycolysis and gluconeogenesis.
X-ray structures of PEPCK provide insight into the structure and the mechanism of PEPCK enzymatic activity. The mitochondrial isoform of chicken liver PEPCK complexed with Mn 2+, Mn2+-phosphoenolpyruvate (PEP), and Mn 2+-GDP provides information about its structure and how this enzyme catalyzes reactions. [10]
An American-style 15×15 crossword grid layout. A crossword (or crossword puzzle) is a word game consisting of a grid of black and white squares, into which solvers enter words or phrases ("entries") crossing each other horizontally ("across") and vertically ("down") according to a set of clues. Each white square is typically filled with one ...
Phosphoenolpyruvate carboxylase (also known as PEP carboxylase, PEPCase, or PEPC; EC 4.1.1.31, PDB ID: 3ZGE) is an enzyme in the family of carboxy-lyases found in plants and some bacteria that catalyzes the addition of bicarbonate (HCO 3 −) to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate and inorganic phosphate: [1]
Many Enterobacteriaceae, including E. coli, have two isoforms of pyruvate kinase, PykA and PykF, which are 37% identical in E. coli (Uniprot: PykA, PykF).They catalyze the same reaction as in eukaryotes, namely the generation of ATP from ADP and PEP, the last step in glycolysis, a step that is irreversible under physiological conditions.
Taking this one stage further, the clue word can hint at the word or words to be abbreviated rather than giving the word itself. For example: "About" for C or CA (for "circa"), or RE. "Say" for EG, used to mean "for example". More obscure clue words of this variety include: "Model" for T, referring to the Model T.
A 15x15 lattice-style grid is common for cryptic crosswords. A cryptic crossword is a crossword puzzle in which each clue is a word puzzle. Cryptic crosswords are particularly popular in the United Kingdom, where they originated, [1] as well as Ireland, the Netherlands, and in several Commonwealth nations, including Australia, Canada, India, Kenya, Malta, New Zealand, and South Africa.
Josep "Pep" Guardiola Sala (Catalan pronunciation: [ˈpɛb ɡwəɾðiˈɔlə]; [3] [4] [5] born 18 January 1971) is a Catalan professional football manager and former player from Spain who is the manager of Premier League club Manchester City. [6]