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Avizo (pronounce: ‘a-VEE-zo’) is a general-purpose commercial software application for scientific and industrial data visualization and analysis.. Avizo is developed by Thermo Fisher Scientific and was originally designed and developed by the Visualization and Data Analysis Group [1] at Zuse Institute Berlin (ZIB) under the name Amira.
Amira (ah-MEER-ah) is a software platform for visualization, processing, and analysis of 3D and 4D data. It is being actively developed by Thermo Fisher Scientific in collaboration with the Zuse Institute Berlin (ZIB), and commercially distributed by Thermo Fisher Scientific — together with its sister software Avizo.
In cryo-sectioning, frozen blocks of cells or tissue are sectioned into thin samples with a cryo-microtome. [11] In FIB-milling, plunge-frozen samples are exposed to a focused beam of ions, typically gallium , that precisely whittle away material from the top and bottom of a sample, leaving a thin lamella suitable for cryoET imaging.
More than 50 years ago, MSI was introduced using secondary ion mass spectrometry (SIMS) to study semiconductor surfaces by Castaing and Slodzian. [5] However, it was the pioneering work of Richard Caprioli and colleagues in the late 1990s, demonstrating how matrix-assisted laser desorption/ionization (MALDI) could be applied to visualize large biomolecules (as proteins and lipids) in cells and ...
In April 2019 the company released a new SMRT Cell with eight million ZMWs, [30] increasing the expected throughput per SMRT Cell by a factor of eight. [31] Early access customers in March 2019 reported throughput over 58 customer run cells of 250 GB of raw yield per cell with templates about 15 kb in length, and 67.4 GB yield per cell with ...
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A bioassay is an analytical method to determine the potency or effect of a substance by its effect on living animals or plants (in vivo), or on living cells or tissues (in vitro). [1] [2] A bioassay can be either quantal or quantitative, direct or indirect. [3] If the measured response is binary, the assay is quantal; if not, it is quantitative ...
As such, it is a very convenient research tool but caution should be used when extrapolating these results to normal cells, and even more, to normal human cells. [ 3 ] [ 4 ] When cultured on tissue culture plastic or hydrogels without cell adhesion motifs MC3T3 cells adopt a polygonal shape without considerable polarization and focal adhesions .