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The Biological Stain commission is an independent non-profit company that has been testing dyes since the early 1920s and issuing Certificates of approval for batches of dyes that meet internationally recognized standards. StainsFile Reference for dyes and staining techniques.
The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. [1] However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods.
The Biological Stain Commission (BSC) is an organization that provides third-party testing and certification of dyes and a few other compounds that are used to enhance contrast in specimens examined in biological and medical laboratories.
In immunohistochemical techniques, there are several steps prior to the final staining of the tissue that can cause a variety of problems. It can be strong background staining, weak target antigen staining and presence of artifacts. It is important that antibody quality and the immunohistochemistry techniques are optimized. [16]
Biological Stains (1925) History of Staining (1933; 1948) Staining Procedures (1944–55; 1960) Manual of Microbiological Methods (1957) Harold Joel Conn (May 29, 1886 – November 10, 1975) [ 1 ] was an American agricultural bacteriologist , known for his work on soil microbiology and bacterial staining techniques.
In the life sciences fluorescence microscopy is a powerful tool which allows the specific and sensitive staining of a specimen in order to detect the distribution of proteins or other molecules of interest. As a result, there is a diverse range of techniques for fluorescent staining of biological samples. [citation needed]
This technique is known as Wright's stain. [19] [20] Gustav Giemsa's name has also become associated with the stain as he is credited with publishing a useful formulation and protocol in 1902. [13] [6] [26] Giemsa attempted to use combinations of pure dyes rather than polychromed methylene blue solutions which are highly variable in composition.
FISH can also be used to compare the genomes of two biological species, to deduce evolutionary relationships. A similar hybridization technique is called a zoo blot. Bacterial FISH probes are often primers for the 16s rRNA region. FISH is widely used in the field of microbial ecology, to identify microorganisms.